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Gene expression data of glucocorticoid resistant and sensitive acute lymphoblastic leukemia cell lines
GEO
Data Resource:  GEO
Point of Contact:  
Johannes Rainer,  
Project

About This Dataset
Glucocorticoids (GCs) cause apoptosis and cell cycle arrest in lymphoid cells and constitute a central component in the therapy of lymphoid malignancies, most notably childhood acute lymphoblastic leukemia (ALL). PFKFB2 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-2), a kinase controlling glucose metabolism, was identified by us previously as GC response gene in expression profiling analyses performed in children with ALL during initial systemic GC mono-therapy. Since deregulation of glucose metabolism has been implicated in apoptosis induction, this gene and its relatives PFKFB1, 3, and 4 were further analyzed. Expression analyses in additional ALL children, non-leukemic individuals and leukemic cell lines confirmed frequent PFKFB2 induction by GC in most systems sensitive to GC-induced apoptosis, particularly in T-ALL cells. The 3 other family members, in contrast, were not or weakly expressed (PFKFB1 and 4) or not induced by GC (PFKFB3). Conditional PFKFB2 over-expression in the CCRF-CEM T-ALL in vitro model revealed that its 2 splice variants (15A and 15B) did not have any detectable effect on survival or cell cycle progression. Moreover, neither PFKFB2 splice variant significantly affected sensitivity to, or kinetics of, GC-induced apoptosis. Our data suggest that, at least in the model system investigated, PFKFB2 is not an essential upstream regulator of the anti-leukemic effects of GC.
Core Data Elements
Number of Cases
13
Case Sex
Female (7); Male (6)
Case Age At Diagnosis
0 to 4 years (5); 5 to 9 years (3); 10 to 14 years (2); 15 to 19 years (3)
Case Race
Not Reported (7)
Case Ethnicity
Not Reported (13)
Case Disease Diagnosis
Acute Lymphoblastic Leukaemia (13)
Case Tumor Site
Peripheral Blood (13)
Additional Data Elements
GEO STUDY IDENTIFIER
Grant Information
W1101
Austrian Science Fund (FWF)
Published In
https://doi.org/10.1186/1471-2407-10-638
Charts
Female (7); Male (6)
7Female